上海419论坛,上海龙凤419,爱上海 – Powered by Teri Savorn!

Tag Archive: 419sh龙凤

Guardiola keen to stay at City beyond current deal

first_imgManchester City manager Pep Guardiola has revealed he is willing to stay at the club beyond his current contract.The Spanish gaffer has dropped hints he could be willing to extend his stay with the Premier League champions beyond his current deal which ends in 2021, despite suggesting earlier that he had a long-term desire to manage a national team.Guardiola said, according to France24:“I have a young squad, good facilities — I have everything. I have protection, my family is doing well in Manchester.”norwich city, manchester city, premier leagueReport: City are stunned by Norwich George Patchias – September 14, 2019 Manchester City was stunned by Norwich City in todays Premier League clash.Much has been made in recent days of the potential impact of Aymeric…“I don’t rule anything out in terms of my contract. We will see. This is day by day. In two months, football can change every impression you have right now.”“You can lose the locker room, you can see the players don’t follow you anymore and make bad decisions. It’s going well and I’ll maybe stay longer. I don’t know.”Manchester City and Guardiola travel to the London Stadium to face West Ham on Saturday, as they look to maintain their unbeaten start to the season.last_img read more

NOW HIRING 50 New Job Openings In Wilmington Week of April 14 2019

first_imgWILMINGTON, MA — Below are job listings previously published on Wilmington Apple during the week of April 14, 2019:Full-Time Physical Therapist at Wilmington Public SchoolsFull-Time Customer Service Representative at D.B. RobertsFull-Time Commissioning Manager at Limbach Facility ServicesFull-Time/Part-Tiem Member Services Representative at Planet FitnessFull-Time Warehouse Associate at West Springfield Auto Parts Inc.Full-Time Warehouse Stockroom Associate at NAPA Auto PartsFull-Time Delivery Driver at AFR Furniture RentalFull-Time Crew Chief Technician at SERVPROFull-Time Delivery Driver at FedEx WarehouseFull-Time Senior Mechanical Engineer at AmetekFull-Time Senior Research Scientist at EnergetiqFull-Time Sr. Quality Regulatory Compliance Engineer at EnergetiqFull-Time Office Administrator at EnergetiqFull-Time Director of Engineering at EnergetiqFull-Time Product Support Engineer at EnergetiqPart-Time Temporary Driver at FedExPart-Time Nanny For 1 Wilmington ChildFull-Time CDL Relief Driver at Pepsi Co.Full-Time Landscape Maintenance Crew Leader at Garrick-SantoFull-Time/Part-Time Pizza Counter/Register Help at Wilmington House of PizzaFull-Time Marketing Specialist at Charles River LabsFull-Time Associate Director Marketing at Charles River LabsFull-Time Product Manager (Discovery Oncology) at Charles River LabsFull-Time AV Rack Builder at Red ThreadFull-Time National Accounts Project Coordinator at Standard ElectricPart-Time Inside Sales Associate at Superior SealcoatPart-Time Cashier at K1 SpeedFull-Time Cargo Van Driver at OptimaPart-Time Babysitter For Wilmington TwinsFull-Time Entry Level Sales at Capital Carpet & Flooring SpecialistsFull-Time Driver at West Springfield Auto PartsFull-Time Counter Sales at West Springfield Auto PartsPart-Time Apparel & Accessories Team Member at TargetPart-Time Electronics Team Member at TargetFull-Time Overnight Custodian at Planet FitnessFull-Time Overnight Closer at Planet FitnessFull-Time HVAC Service Technician at InLine Mechanical LLCFull-Time Member Service Representative at Align Credit UnionPart-Time Certified Personal Trainer at NA FitnessPart-Time Sandwich Artist at SubwayFull-Time Class D Delivery Driver at J. Polep Distribution ServicesFull-Time Administrative Assistant/Receptionist at O’Brien ConstructionFull-Time 1st Shift Production Technician at CutisPharmaFull-Time HR Business Partner at Charles River LabsFull-Time Service Manager/Technician at Ritchie & SonsFull-Time Deployment Specialist at Locus RoboticsFull-Time Deployment Project Manager at Locus RoboticsFull-Time Sales Executive at Locus RoboticsFull-Time Inbound Sales Specialist at Parterre Flooring SystemsFull-Time Customer Service/Technician at PALMS Environmental: PALMS Environmental, located in Wilmington, is a rental company serving the Environmental Consultant and Engineering community.  We offer a full line of monitoring and sampling equipment for water, soil and air since 1999.  We are looking for an individual who can maintain our equipment, interact with customers, perform deliveries and wear many hats as required in a small business. No experience is required, we will train you.  This is a full time position and mother’s hours are acceptable. PALMS Environmental offers an excellent working environment, and competitive salary with benefits. Interested candidates should contact: Dennis Hart, palmsenv@yahoo.com(NOTE: Wilmington businesses — Feel free to send me your job postings at wilmingtonapple@gmail.com.)Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email wilmingtonapple@gmail.com.Share this:TwitterFacebookLike this:Like Loading… RelatedNOW HIRING: 50 New Job Openings In Wilmington (Week of August 18, 2019)In “Business”NOW HIRING: 60 New Job Openings In Wilmington (Week of July 14, 2019)In “Business”NOW HIRING: 60 New Job Openings In Wilmington (Week of August 4, 2019)In “Business”last_img read more

Mollie Dickerson Appointed Guidance Curriculum Team Leader For Wilmington High Wilmington Middle School

first_imgWILMINGTON, MA — Below is a press release from Superintendent Dr. Glenn Brand, announcing the appointment of Ms. Mollie Dickerson as Guidance Curriculum Team Leader:Over the course of the last number of weeks we have launched a search for a permanent Curriculum Team Leader (CTL) for our Guidance Department as we currently have in an interim in the role. Ms. Christine Murray, Coordinator of Behavioral Health and Social Emotional Support coordinated the search with a screening committee that included the following individuals:Jeanette Quirk, Assistant Principal, MSChristopher Phillips, Assistant Principal, HSMaureen O’Mara, HS GuidanceJessica Rugo, HS GuidanceJulie Norton, MS GuidanceMichael Maloney, HS Social Studies TeacherLisa Costantiello, Parent/SEPAC Co‐ChairI want to extend to these individuals my thanks for their time and commitment to this important process.The screening committee reviewed a total of twenty‐two (22) applications from both inside and outside the district and selected a total of five (5) candidates to interview during the week of May 20th. Following the work of the district a total of three (3) candidates were advanced as finalists who then participated in interviews with Ms. Linda Peters, Principal of the Wilmington High School, Mr. Kevin Welch, Interim Principal of the Wilmington Middle School and Ms. Alice Brown‐Legrand, Director of Student Support Services during the week of May 27th.It is with pleasure that I announce the appointment of Ms. Mollie Dickerson as the Guidance Curriculum Team Leader for the Wilmington Public Schools. This position is a key leadership role in leading the school guidance departments and staff in both the middle and high school.Ms. Dickerson was appointed as the Interim Guidance CTL for the 2018‐2019 school year here in WPS. She is credited with several new initiatives in this role such as arranging for Therapy Dog visits, expanding the guidance curriculum to include more workshops for students, serving on the NEASC Steering Committee, and planning and hosting our first Future Teacher Signing Day.Ms. Dickerson completed three years as a guidance counselor at Wilmington High School prior to her appointment this year. Ms. Dickerson is a graduate of Brigham Young University where she received Bachelors of Science in Psychology and English. She received her Masters of Education in Prevention Science and Practice and in Adolescent School Counseling as well as her Certificate of Advanced Studies in School Guidance Counseling from Harvard University.Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email wilmingtonapple@gmail.com.Share this:TwitterFacebookLike this:Like Loading… RelatedNAME CHANGE: ‘Guidance Counselors’ Now Referred To As ‘School Counselors’ In WilmingtonIn “Education”NEW ROLES FOR 3 FAMILIAR EDUCATORS: Shelia Burke, Leanne Ebert & Kevin Welch Named To Leadership PositionsIn “Education”50+ PERSONNEL CHANGES: Who Joined & Who Left Wilmington Public Schools Over The Summer?In “Education”last_img read more

2018 A year of creepy AF smart home headlines

first_img See All Google’s efforts were impressive, but Duplex didn’t sit right with all of us. “In this age of disinformation, where fake news thrives and the public has trust issues with technology, Google designed a machine that can deceive humans,” wrote CNET’s Bridget Carey, who also voiced concerns over the fact that Google’s Assistant never identified itself as a robot. “Gosh, what could go wrong?”Two days after that editorial, Google told CNET explicitly that it will launch Duplex with “disclosure built-in” for better transparency.An Amazon Key in-home delivery: not as creepy as we expected! Tyler Lizenby/CNET Then there’s Amazon Key, which looks to leverage smart lock tech at your front door to let delivery people drop Amazon packages off inside your home. The Amazon Key Home Kit even includes an Amazon Cloud Cam to let you watch the delivery in real time right from your phone (more on cameras in just a bit, by the way).The idea of granting a stranger authorized access to your home was unnerving for many of us — but in the end, Amazon Key wasn’t nearly as creepy as we expected. In fact, the most we ever saw of the delivery people was an arm reaching inside the cracked door to drop a package off just inside. From Megan Wollerton’s recap:”While I started out hesitant about in-home delivery, I’d be comfortable enough to use it going forward (and to recommend it to frequent Amazon customers) — as long as I can keep an eye on whoever’s delivering the packages from my phone.”Camera creep2018 might be the year when the mainstream smart home opened its eyes, with a flood of new camera-equipped products and services designed to help us keep watch over our homes or stay in touch with loved ones. Early in the year, for instance, Amazon bought the home security startup Ring and its popular video doorbell. Now, a new app for Ring users called Neighbors lets you share, view and comment on local crime activity, complete with video clips from Ring cameras and doorbells in your community.The Nest Hello video doorbell is one of a growing number of smart home products using cameras equipped with facial-recognition technology. Chris Monroe/CNET That’s an appealing pitch to some, but consider the company’s recent patent applications focused on facial recognition. Each considers ways to use Ring cameras to identify “suspicious” people (convicted felons, sex offenders, etc.), then automatically alert law enforcement. That raised fresh surveillance state concerns from the American Civil Liberties Union.”Amazon is dreaming of a dangerous future,” the ACLU’s Jacob Snow said in a statement, “with its technology at the center of a massive decentralized surveillance network, running real-time facial recognition on members of the public using cameras installed in people’s doorbells.”We expect to see more uses of facial recognition technology in the coming year, including from cameras that are already equipped with the feature, like Google’s Nest Hello video doorbell. In other words, don’t expect this issue to fade from sight.And it’s not just the front door — from night-vision security cameras to connected baby monitors, people are parking cameras inside their homes, too. Doing so might require you to wrap your head around some potential privacy vulnerabilities. For instance, earlier this year, researchers from Kaspersky Lab warned that hackers could turn your own cameras against you by spying on you, or by fooling you with a duplicate, “cloned” feed. Your next vacation rental might have security cameras inside, too, which raises concerns about whether or not you could be filmed without realizing it during a weekend getaway.  That brings us to smart displays, a new smart home category that promises to bring even more cameras into people’s kitchens and living rooms. Amazon was first into the space with the Echo Show and Echo Spot, and Google soon followed suit with a suite of its own Google Assistant-powered touchscreens, including the Lenovo Smart Display and the JBL Link View. Tellingly, Google opted not to include a camera in its flagship, first-party smart display, the Google Home Hub.Of course, there’s another smart display worth mentioning. In fact, it gets the next section of this post all to itself.fl-portal-10 Chris Monroe/CNET Smart home hubris from FacebookThere was a collective “you’ve gotta be f***ing kidding me” from many of us who write about tech when Facebook, in the midst of scandal after scandal after scandal over the misuse of user data, unveiled the new Facebook Portal in-home video chatting devices. Because sure, why not let a transparency-challenged company that’s been — at best — wildly irresponsible with user data bring person-tracking cameras and always-listening microphones into your living room? They promise they’ll behave! Tags CNET Smart Home Aug 31 • The best coffee grinders you can buy right now More recently, a judge in New Hampshire ordered Amazon to hand over the Alexa audio of a user accused of two counts of first degree murder. For now, Amazon is fighting the ruling.”Amazon will not release customer information without a valid and binding legal demand properly served on us,” an Amazon spokesperson told CNET. “Amazon objects to overbroad or otherwise inappropriate demands as a matter of course.”   Concerns about silent, “ultrasonic” audio attacks also raised our collective assistant anxiety this year, with researchers claiming that the most popular voice platforms were vulnerable to audio cues at frequencies too high for humans to hear. Researchers at UC Berkeley claim they were even able to fool Mozilla’s open-source DeepSpeech voice-to-text engine by hiding ultrasonic audio cues within brief snippets of music.Maybe most disconcerting — to date, none of the major tech companies responsible for these voice platforms have denied that attacks like these are possible.google-duplex-orens-hummus-demo-2-2Enlarge ImageWith Google Duplex, Google Assistant will call restaurants and salons to make reservations on your behalf. Google’s demo was one of the most convincingly life-like AI use-cases we’d ever seen. James Martin/CNET Big moonshots, big questions2018 saw some notable new ideas about where smart home tech may be headed — and some of those ideas raised a lot of questions.Let’s start with Google Duplex, the search giant’s effort to let the artificially intelligent Google Assistant make phone calls on your behalf. Google touted the feature as a way for the Assistant to book things like haircuts and dinner reservations — and the demo was pretty mind-blowing. Watch for yourself below: 1 Consumer Product Safety Commission asks: How dangerous is the internet of things? Here’s what Congress wants to know about Amazon’s Echo Dot for kids Senators call on FTC to investigate smart TVs tracking viewers’ data Your smart air conditioner could help bring down the power grid, researchers say California governor signs country’s first IoT security law Comment Google’s Duplex: AI that makes you think you’re talking to a human Voice of concern: Smart assistants create new openings for hackers Watch Google Assistant fire a gun Robot or human? Google Assistant will leave you guessing And hey, amid escalating scrutiny, perhaps they will. “We have a responsibility to protect your data,” Facebook CEO Mark Zuckerberg wrote in a March 21 statement following the company’s Cambridge Analytica data-mining scandal. “And if we can’t then we don’t deserve to serve you.”Just keep in mind that since then, Zuckerberg’s company has come under continued fire for not doing enough to deal with a flood of fake news and Russian trolls on its platform during the 2016 US election. In July of this year, the company admitted to sharing user information with hardware and software partners even after claiming to discontinue the practice in 2015. Just this week, the New York Times reported that Facebook used “special arrangements” like those even more than the company initially disclosed — namely, to give Microsoft’s Bing search engine access to the names of all Facebook users’ friends without consent, and to allow Netflix and Spotify to read Facebook users’ private messages.Netflix denies that it ever accessed people’s private messages on Facebook, or asked for the ability to do so. Spotify didn’t immediately return a request for comment.At any rate, we are well past the point of taking Facebook’s word for anything when it comes to protecting your private data. But, if early market indications showing lots of interest in smart displays this holiday buying season are correct, that’s exactly what some people might be doing. That, or they’ve been drawn in by Facebook’s admittedly slick user interface — enough so to shrug off some extremely valid privacy concerns.After the year we’ve had, maybe that’s what creeps me out the most. reading • 2018: A year of creepy AF smart home headlines Microphone mishaps (no laughing matter)Things typically get a bit quiet in tech during the months following January’s big CES expo in Las Vegas. This year, a mysterious and disconcerting bout of laughter echoed out of the silence.The cackle in question came from Alexa, when users of Amazon’s popular voice assistant began sharing clips of eerie, unprompted laughter emitting from their Echo devices. Once the phenomenon began to trend on Twitter, Amazon confirmed the issue and told us it was looking into a fix.2018 saw a number of stories about Echo devices acting strangely. Chris Monroe/CNET “In rare circumstances, Alexa can mistakenly hear the phrase ‘Alexa, laugh,'” the company ultimately explained. “We are changing that phrase to be ‘Alexa, can you laugh?’ which is less likely to have false positives, and we are disabling the short utterance ‘Alexa, laugh.’ We are also changing Alexa’s response from simply laughter to ‘Sure, I can laugh’ followed by laughter.”In another Alexa headache this May, a family in Oregon claimed that their Echo device recorded audio of a private conversation and sent it out to a random contact without warning. Amazon’s explanation? It was the Alexa equivalent of a butt-dial.”[The] Echo woke up due to a word in background conversation sounding like ‘Alexa,'” an Amazon spokesperson told CNET. “Then, the subsequent conversation was heard as a ‘send message’ request. At which point, Alexa said out loud, ‘To whom?’ At which point, the background conversation was interpreted as a name in the customer’s contact list. Alexa then asked out loud, ‘[contact name], right?’ Alexa then interpreted background conversation as ‘right.'””As unlikely as this string of events is, we are evaluating options to make this case even less likely,” Amazon added.Still, accidents happen. Just today, Reuters reports that Amazon mistakenly sent the wrong files to an Alexa user in Germany who had requested his Echo recordings. As a result, he was able to download and access 1,700 recordings from another user’s household. Per Reuters, Amazon called this an isolated incident, and the result of human error.Assistant anxietyAmazon also found itself navigating potential legal battles over user privacy when Alexa became an apparent witness to not one, but two separate cases of homicide. The first came to a conclusion in March when Amazon agreed to hand over the Alexa audio recordings of a user in Bentonville, Arkansas, who stood accused of of first-degree murder, but only after that user consented to the release. Aug 31 • Alexa can tell you if someone breaks into your house 2018 is just about over, and it’s common for tech reporters to dig back into their beats to try and sum up the year’s news. And, as the CNET Smart Home team took a look back for our own year in review, there was one takeaway we just couldn’t escape:The smart home was… kinda creepy this year.To wit, there were evil cackles from Alexa, fake phone calls from Google Assistant, and concerns a-plenty about the connected cameras and microphones filling our homes. That’s not to mention the well-founded fears about the mass amount of data that these devices are asking to be trusted with (looking right at you, Facebook Portal. No thanks.)So, yeah, if the smart home had you a bit creeped out in 2018, I can’t say I blame you. Here’s a look back at the stories that probably played a role. Tough questions for the smart home in 2018 So Alexa decided to laugh randomly while I was in the kitchen. Freaked @SnootyJuicer and I out. I thought a kid was laughing behind me. pic.twitter.com/6dblzkiQHp— CaptHandlebar (@CaptHandlebar) February 23, 2018 Aug 30 • Battling bot vacs: iRobot Roomba S9+ vs Neato Botvac D7 Connected 4:25 Aug 31 • Best smart light bulbs for 2019 (plus switches, light strips, accessories and more) Now playing: Watch this: Share your voice 2018 in creepy assistants CNET Smart Home • Smart Homelast_img read more

Heres how Salman Khan pranked Raveena Tandon on Nach Baliye 9 sets

first_imgRaveena Tandon, Salman KhanInstagramSalman Khan and Raveena Tandon go back a long way. She made her debut in Bollywood with Salman in 1991 film Patthar Ke Phool and then went on to star in Andaz Apna Apna which became one of the cult movies of the industry. So it was pretty obvious for Salman to play a prank on Raveena when they reunited on the sets of Nach Baliye 9.The Dabangg Khan told the entire crew of Nach Baliye 9 that he knows Raveena since the last 120 years and spoke non-stop on the set.”He (Salman) told everyone that he knows me since the last 120 years. There’s a comfort factor with him. We spoke non-stop on the set, but when the episode was telecast, I realised that they had edited out a major chunk due to time constraints. We then told each other, ‘Why did we talk so much?'” Raveena Tandon told Mid-Day.Raveena, along with Suniel Shetty, was recently featured in the recreated version of their hit song Sheher Ki Ladki from the 1996 film Rakshak. The video of the remake version also features Diana Penty and Badshah grooving to the song.last_img read more

Road crashes kill 16 in 5 dists

first_imgAt least 16 people were killed in road accidents in Gopalganj, Sirajganj, Tangail, Rajshahi and Pabna districts on Thursday, says UNB.Six of them were killed in Gopalganj while four in Sirajganj and two each in Tangail, Rajshahi and Pabna.In Gopalganj, a bus rammed a microbus at Gerekhola in Kashiani upazila in the morning, killing six people, including five members of a family.Five of the deceased were identified as Halim Akon, a Saudi expatriate, his wife Asma Khatun, their two sons — Sujon and Shihab — and Halim’s brother-in-law Babul Hawladar, of Sharankhola upazila in Bagerhat district.Saidur Rahman Khan, police super of Gopalganj, said the Dhaka-bound bus of ‘Sheba Greenline Paribahan’ crashed into the microbus coming from the opposite direction around 9:45am, leaving the six microbus passengers dead on the spot.The family met the tragic end of their lives while returning home after receiving Halim, who came to Bangladesh from Saudi Arabia in the morning, at Hazrat Shahjalal International Airport in the capital.In Sirajganj, four people, including three members of a family, were killed and two others injured when a bus hammered a microbus on Dhaka-Bogra highway at Ghurka Beltala in Raiganj upazila in the early hours.The deceased were identified as Mili Begum, 35, wife of Harun-or-Rashid, their son Sagar, 13, Mili’s father-in-law Solaiman, 65, of Kahalu upazila, and Abdul Khalek, 32, the microbus driver hailing from Dupchachia upazila of Bogra district.Abdul Gani, a sergeant of Hatikumrul Highway Police, said the Kurigram-bound bus of ‘Dhanshiri Paribahan’ hit the microbus around 12:45am, leaving its four inmates killed on the spot and two others injured.In Tangail, a Jamalpur-bound microbus ran two women over at Pathanbari of Dhanbari upazila in the morning while they were on a morning walk along Madhupur-Jamalpur road, killing them on the spot.The deceased were Reba Begum, 32, wife of Babul Bakul, and Shahaton Begum, 50, wife of Abdul Mazid, of the area.Mujibur Rahman, officer-in-charge of Dhanbari police station, said the microbus driver fled the scene leaving his vehicle behind.In Rajshahi, a young man and his nephew were killed as a truck hit their motorbike on Rajshahi-Dhaka highway at Pollapukur intersection in Puthia upazila at noon.The deceased were Tushar Hossain, 22, son of Abdul Latif, and Shahin Ali, 20, son of Nazmul Islam of Ramchandrapur Kedurmor area of the city.Police said the truck crashed into the bike of Tushar when he along with his nephew Shahin was going to Natore, leaving the duo dead on the spot.In Pabna, two motorcyclists were killed as their bike skidded on the Dashuriya-Muladuli road in Muladuli area of Ishwardi upazila in the afternoon.The deceased were Asharful Islam, 25, son of Hasibur Rahman, resident of Arjopara village, and Shipon Hossain, 23, son of Khalilur Rahman, resident of Dori Nazirpur village of Atgharia upazila.Sub-inspector Ganesh Chandra Mandal, in-charge of Pakshi highway police outpost, said the motorcycle skidded off the road in front of a sugarcane field when its driver tried to put the brake, leaving the duo dead on the spot.last_img read more

Saudi king crown prince condole Chawkbazar fire deaths

first_imgSaudi Arab’s king Salman bin Abdulaziz Al Saud and crown prince Mohammed bin Salman bin Abdulaziz Al Saud. Photo: AFPCustodian of the Two Holy Mosques king Salman bin Abdulaziz Al Saud and crown prince Mohammed bin Salman bin Abdulaziz Al Saud have offered condolences to the families and friends of the victims of the tragic fire of Old Dhaka that claimed 67 lives, reports UNB.They sent separate condolence messages to president M Abdul Hamid as a fire ripped through buildings in the capital, resulting in deaths and injuries, according to the Bangladesh embassy in Riyadh.In his message, the King said: “We’ve learned of the news of the blaze took hold in the Chawkbazar area in Dhaka and resulted in a considerable number of deaths and injuries.”“In my own name and on behalf of the government and people of the Kingdom of Saudi Arabia, we express our sincere condolences to you, the families of victims and the people of People’s Republic of Bangladesh, wishing the wounded a speedy recovery,” the message reads.In his message, the crown prince said, “I’ve learned of the news of the blaze took hold in the Chawkbazar area in Dhaka and resulted in a considerable number of deaths and injuries. I express my sincere condolences to Your Excellency and the families of victims, wishing the wounded a speedy recovery.”last_img read more

Researchers unravel the elusive source of sulfur in an antibiotic

first_imgBall-and-stick model of lincomycin. Credit: Jynto/Wikipedia Journal information: Nature Scientists study how marine bacteria release cloud-making compound Citation: Researchers unravel the elusive source of sulfur in an antibiotic (2015, January 15) retrieved 18 August 2019 from https://phys.org/news/2015-01-unravel-elusive-source-sulfur-antibiotic.html Explore further More information: Metabolic coupling of two small-molecule thiols programs the biosynthesis of lincomycin A, Nature (2015) DOI: 10.1038/nature14137AbstractLow-molecular-mass thiols in organisms are well known for their redox-relevant role in protection against various endogenous and exogenous stresses1, 2, 3. In eukaryotes and Gram-negative bacteria, the primary thiol is glutathione (GSH), a cysteinyl-containing tripeptide. In contrast, mycothiol (MSH), a cysteinyl pseudo-disaccharide, is dominant in Gram-positive actinobacteria, including antibiotic-producing actinomycetes and pathogenic mycobacteria. MSH is equivalent to GSH, either as a cofactor or as a substrate, in numerous biochemical processes4, most of which have not been characterized, largely due to the dearth of information concerning MSH-dependent proteins. Actinomycetes are able to produce another thiol, ergothioneine (EGT), a histidine betaine derivative that is widely assimilated by plants and animals for variable physiological activities5. The involvement of EGT in enzymatic reactions, however, lacks any precedent. Here we report that the unprecedented coupling of two bacterial thiols, MSH and EGT, has a constructive role in the biosynthesis of lincomycin A, a sulfur-containing lincosamide (C8 sugar) antibiotic that has been widely used for half a century to treat Gram-positive bacterial infections6, 7, 8, 9. EGT acts as a carrier to template the molecular assembly, and MSH is the sulfur donor for lincomycin maturation after thiol exchange. These thiols function through two unusual S-glycosylations that program lincosamide transfer, activation and modification, providing the first paradigm for EGT-associated biochemical processes and for the poorly understood MSH-dependent biotransformations, a newly described model that is potentially common in the incorporation of sulfur, an element essential for life and ubiquitous in living systems.center_img As Melancon notes, there are a host of molecules vital for biological processes that have sulfur as a component—their metabolic origins can be predicted as a matter of course. Sulfur atoms on the other hand have been much more difficult to predict. Overcoming such obstacles for some molecules could have benefits such as helping to develop new medicines. In this new effort the researchers set their sights on lincomycin A, an antibacterial agent that has been used to treat Gram-positive bacterial infections for many years.To find the sulfur source, the researchers used modern whole genome sequencing techniques to work backwards through likely pathways looking to isolate intermediaries that tended to build up as genes were disrupted. Doing so led to the discovery that disruption of lmbE led to intermediates that looked a lot like lincomycin—that led them to study lambV, a gene that also shows up in lincomycin, which led to the discovery of another intermediary. The team also tested other enzymes and found a way to prove that there were at least three of them that incorporated sulfur into parts of the antibiotic, which was the original goal, of course. More specifically, they found that two bacterial thiols played a constructive role in the biosynthesis of lincomycin A. The team then went further, isolating the materials that were produced due to the genes involved, along with the functions of enzymes that were part of the process.Work done by the team should help provide a pathway for other researchers attempting to find the source of sulfur atoms in other molecules, perhaps leading to new types of materials for use in a variety of medical purposes, from antibiotics to cancer fighting agents. © 2015 Phys.org (Phys.org)—A team of researchers working at the Chinese Academy of Sciences in Shanghai has for the first time found the source of sulfur atoms in lincomycin A, a lincosamide widely used as an antibiotic agent. In their paper published in the journal Nature, the team describes their research efforts and results. Charles Melancon with the University of New Mexico outlines and comments on the work done by the team in the same journal issue. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more

Fiction returned to the fore in 2017

first_imgFiction was almost lost from Indian publishing at about this time last year. With about three non-fiction titles releasing for every novel, it was clear who ruled the roost in 2016 – just as it did in 2015. In 2017, however, book lovers had a lot to chew upon as some really strong novels made their mark both in the market and among readers. Their numbers again may have been fewer than non-fiction, but it was the utter magic of some strong novels that stole the show. Also Read – Add new books to your shelfCall it the return of Arundhati Roy or the magic of Jeet Thayil, but most readers would agree that 2017 has been a fantastic year for Indian publishing when one looks at the fiction space.Poulomi Chatterjee, the dynamic Editor-in-Chief and Publisher at Hachette India, agreed that 2017 was a good year for fiction in Indian publishing but also highlighted that non-fiction continued to rule the market. Subroto Bagchi’s “Sell”, Muhammad Yunus’ “A World of Three Zeros”, Alan Rosling’s “Boom Country” and Ankit Lal’s “India Social” were among the better-selling books from Hachette India this year. Also Read – Over 2 hours screen time daily will make your kids impulsive”The year 2017 has seen a good mix of releases across genres from all publishers in India. While non-fiction categories have remained the better-selling ones, there have certainly been strong fiction titles that have found their readership and made their mark. Publishers will always strive to publish strong fiction because this, after all, is the soul of a publishing list,” Chatterjee told in an interview.She elaborated that on the “great fiction list across a range of genres” that Hachette published this year are Pradeep Sebastian’s dream of a book on books, “The Book Hunters of Katpadi”; Arunava Sinha’s excellent translation of Moti Nandy’s short stories “Kick-Off”; Sanjay Bahadur’s kick-ass military thriller “Bite of the Black Dog”; Komal Bhanver’s “The Mauryan”, featuring the story of a young Ashoka; and Swati Kaushal’s delightful year-end read, “A Few Good Friends”. “And that’s just a few – there’s much more fiction to come next year, all of it exciting,” Chatterjee added.Having published some of the most talked-about literary novels in 2017 like “The Ministry of Utmost Happiness” by Arundhati Roy, “Exit West” by Mohsin Hamid, “The Golden House” by Salman Rushdie, “A State of Freedom” by Neel Mukherjee, “A Life of Adventure and Delight” by Akhil Sharma and “We That Are Young” by Preti Taneja, Penguin Random House India Editor-in-Chief, Meru Gokhale, said that 2017 was “the year of literary fiction” for his company.”Each of these books won critical acclaim and pretty much all of them were seen on award listings and bestseller charts. Above and beyond all of these quantifiable measures, what these books did was to capture the imagination in a year that saw tumultuous political change across the globe.””I think every publisher in India will agree that, in times like these, of alternative truths and cultural shifts, fiction – at least good fiction – grants us insight and escape, a way of understanding our dark new age, and an unapologetically provocative worldview,” Gokhale told.While most publishers agreed that 2017 was a good year for Indian fiction, Udayan Mitra, Publisher – Literary, Harper Collins India, pointed out that Indian fiction has been really strong over the past few years. He, however, agreed that there were some strong novels in 2017.”I think it’s been a great year for literary publishing – we’ve seen some very good books being published, and more are on their way. I wouldn’t say there was any need to reclaim fiction as such, since Indian literary fiction has been really strong over the past several years. But yes, we have had some very strong novels coming out, and some great new voices making their presence felt too.””What is particularly interesting to me is the way in which fiction writers in India are reacting and responding to the environment that they live and write in – it has brought a sharp edge to many of the books that have come out this year. Another notable trend is the re-emergence of short fiction – the ability of a novelist to tell a good story well in a space of 200 pages or less – which I believe has something to do with the sensibilities of both readers and writers being shaped by the age of social media,” Mitra told in an interview.Other publishing houses that published strong fiction during 2017 include Aleph (“The Book of Chocolate Saints”); Juggernaut (“When I Hit You”; and Niyogi Books (“Without Prejudice”).last_img read more

Protecting Your Tech From Human Sabotage

first_img This hands-on workshop will give you the tools to authentically connect with an increasingly skeptical online audience. Free Workshop | August 28: Get Better Engagement and Build Trust With Customers Now June 1, 2006 Opinions expressed by Entrepreneur contributors are their own. Enroll Now for Free 2 min read All the firewalls, anti-virus software and spam killers in the world won’t save you from poor user behavior. A recent survey of IT managers by security company GreenBordershowed that most of the malicious code they have to clean from computers resulted from mobile users bringing back “souvenirs” or from user behavior, such as downloading bad software.There are ways to help protect your business from human error. “It’s a two-pronged approach: education, and protecting [employees] from themselves. You need to educate [employees] about clicking on e-mail attachments and downloading software, [and] you need to have the appropriate security software on their computers,” says Laura Yecies, general manager of Check Point Software Technologies’ recently acquired Zone Labsdivision, a consumer and SMB network security provider.A security suite should be installed on every laptop your mobile workers use. Employees should be instructed in proper user behavior. Points to mention include not using work e-mail for personal use, not installing peer-to-peer networking software or any other un-authorized programs, and not plugging gadgets like PDAs into work computers. Software is available that allows you to place usage restrictions on your server or block certain sites from employee access at work. A comprehensive technology and internet security policy is a good start, but also be sure to update the policy and keep employees trained and informed.last_img read more

The LowTech Hack Youre Not Prepared For

first_img Free Webinar | Sept. 9: The Entrepreneur’s Playbook for Going Global Register Now » This story appears in the September 2016 issue of Entrepreneur. Subscribe » Growing a business sometimes requires thinking outside the box. We’re inundated with terrifying tales of computer hacking. But seldom discussed is the relatively low-tech act of visual hacking: That’s when a snoop sneaks a peek at or photographs your sensitive information. In a Ponemon Institute experiment conducted on behalf of 3M and the Visual Privacy Advisory Council, an undercover hacker posing as a contractor or part-time worker was able to obtain sensitive info (like log-in credentials) 88 percent of the time. Related: Cyber Security a Growing Issue for Small BusinessHeighten awareness: In the Ponemon Institute study, employees did nothing to stop the undercover operative 70 percent of the time. Be sure to inform your staff of the risks of visual hacking, and have them memorize “the three Rs”: 1. Refrain from sharing key customer or business information with others. 2. Remove such information from business forms and documents where possible. 3. Redact the sensitive information that cannot be removed.Reduce vulnerability: Identify places where confidential materials are stored, such as workstations, printer and fax areas or conference room whiteboards. The more public the workspace, the more tightly you’ll want to lock it down. Place shredders or secured waste containers where only authorized personnel can access them. Keep documents out of plain sight by using printers with a “locked print” option (which requires passcode entry upon pickup) and instituting a clean-desk policy.Related: 10 Questions to Ask When Creating a Cybersecurity Plan for Your BusinessProtect your screens: This is going to sound paranoid, but Burks says it’s for real: If you have computer screens in public areas, position them in a way that makes them hard to snoop on, and use password-­protected screensavers. Privacy filters (films that block side views of your screen) are your friend, especially on laptops or smartphones that employees use outside the office. And consider where visual hacking and computer hacking can intersect: Check that wi-fi security cameras aren’t aimed at confidential information and are protected by strong passwords.  2 min read September 22, 2016last_img read more